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CONTRIBUTION TO FOOD INTEGRITY IN CEREALS SEEDS: A GENETIC APPROACH

seminario 33
Jueves 4 de julio, de 12:00 h a 13:00 h, Aula de Producción chica (2do nivel)

A cargo del Dr. Diego Posik.


Food integrity is an increasing concern around the world. Since the sanction and implementation of food laws to protect the consumers, several methods have been developed to detect and trace adulteration food, including DNA analysis. In the present study, we used a High Resolution Melting real time Post PCR (HRM) analysis and End Point PCR using specie specific primers to evaluate genetic markers as candidates for food authenticity in Chia (Salvia hispanica), Sesame (Sesame indicus) and Flax (Linum usitatissimum) species identification in food containing seeds. Samples seeds of the three species were obtained from grocery stores in Argentina, Mexico and Italy. DNA extraction was a combination between a mechanic lysis of seed and the DNA purification from the grinding material. HRM was performed with primers for the chloroplast ribulose-1,5-bisphosphate (RBCL). The HRM analysis can discriminate between the Chia, Flax and Sesame species individually and its presence in complex food matrix. However, the method cannot be appropriated in matrix food containing a mixture in different proportion of the three species in simultaneous. Whereas, EP PCR, is simple and cost effective alternative approach to the HRM analysis, especially for those samples that has mixtures of more than two different species. Target NGS assays are carrying out in order to contribute to the knowledge of different techniques in the agro food integrity.



Keywords: food authenticity, HRM, Rbcl



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